CST, 18935S, IL-6R alpha/CD126 (E5B3P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IL6R. Validated for WB,IP,IF,F,F. Available in 2 sizes. Highly specific and rigorously validated in-house, IL-6R alpha/CD126 (E5B3P) Rabbit Monoclonal Antibody (CST #18935) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400 Flow Cytometry (Live): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity IL-6R alpha/CD126 (E5B3P) Rabbit Monoclonal Antibody recognizes endogenous levels of total IL-6Rα/CD126 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human IL-6Rα/CD126 protein. Background The IL-6 receptor is a heterodimeric complex that consists of a ligand-binding IL-6 receptor α (IL-6Rα) subunit and a signaling component, gp130 (1). Binding of IL-6 to IL-6Rα results in receptor dimerization with gp130 and subsequent STAT3 activation (1). IL-6Rα is cleaved from the cell surface by ADAM17 (1,2). In humans, soluble IL-6Rα is also generated via alternatively spliced mRNA (1,3). Soluble IL-6Rα binds to IL-6 and can stimulate signaling via membrane bound gp130 in a process known as "trans-signaling" (1). It is through trans-signaling that IL-6 stimulates cells that do not express membrane bound IL-6Rα (1). Alternate Names CD126; CD126 antigen; gp80; HIES5; IL-6 receptor subunit alpha; IL-6R 1; IL-6R subunit alpha; IL-6R-1; IL-6R-alpha; IL-6RA; IL6Q; IL6QTL; IL6R; IL6RA; IL6RQ; interleukin 6 receptor; interleukin 6 receptor alpha subunit; Interleukin-6 receptor subunit alpha; Membrane glycoprotein 80; MGC104991; sIL6R; Soluble interleukin-6 receptor subunit alpha Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 70-100 Source/Isotype: Rabbit IgG