CST, 19017S, MUC16 (IHC125) Mouse Monoclonal Antibody

Monoclonal Antibody for studying MUC16. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, MUC16 (IHC125) Mouse Monoclonal Antibody (CST #19017) is ready to ship. Product Usage Information Western Blotting: 1:1000 IHC Leica Bond: 1:800 - 1:3200 Immunohistochemistry (Paraffin): 1:400 - 1:1600 Storage Supplied in a Tris-based buffer with 1% BSA and less than 0.1% sodium azide. Stable for 24 months when stored at 4°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity MUC16 (IHC125) Mouse mAb recognizes endogenous levels of total MUC16 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with full-length native MUC16 protein. Background Mucins are a family of macromolecules that line and protect the respiratory epithelium from microbes and pollutants in the local environment. Of the family members that are known to date, some are produced in a cell type and tissue-specific manner, suggesting distinct biological roles for members. Some members polymerize after secretion to form gel-like substances that coat the epithelial layer (1). MUC16 is a member of the mucin family, which are high molecular weight, O-glycosylated proteins that play an important role in forming mucous barriers (2) and are found on the apical surface of the epithelia. It contains an extracellular domain at its amino acid terminus, a large tandem repeat, and a transmembrane domain with a short cytoplasmic domain. MUC16 has been linked to lung cancer (3) and ovarian cancer (4). It provides protective, lubricating barriers against particles and infectious agents at mucosal surfaces (5). Alternate Names CA-125; CA125; CA125 ovarian cancer antigen; FLJ14303; MUC-16; MUC16; mucin 16, cell surface associated; Mucin-16; Ovarian cancer-related tumor marker CA125; Ovarian carcinoma antigen CA125 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 200-270 Source/Isotype: Mouse IgG1