CST, 19257S, Asparagine Synthetase (E6C2C) Rabbit Monoclonal Antibody (Pacific Blue™ Conjugate)

Monoclonal Antibody for studying ASNS. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Asparagine Synthetase (E6C2C) Rabbit Monoclonal Antibody (Pacific Blue^™ Conjugate) (CST #19257) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Asparagine Synthetase (E6C2C) Rabbit Monoclonal Antibody (Pacific Blue™ Conjugate) recognizes endogenous levels of total asparagine synthetase protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys467 of human asparagine synthetase protein. Background Asparagine synthetase (ASNS) catalyzes the synthesis of asparagine from aspartate and glutamine. Research studies have shown that intracellular asparagine can suppress apoptosis in a large number of human tumors. In addition, ASNS expression levels have been associated with the progression of gliomas and neuroblastomas in patients (1). Furthermore, acute lymphocytic leukemia cells frequently depend upon serum asparagine for their viability, as they lack ASNS (2). Deprivation of asparagine by L-asparaginase has therefore been developed as a therapeutic treatment for acute lymphocytic leukemia (2,3). In subsets of gastric and hepatic cancers, ASNS promoter hypermethylation correlates with low ASNS expression, sensitizing these cancers to the asparaginase treatment (4). Alternate Names ASNS; ASNSD; asparagine synthetase; asparagine synthetase (glutamine-hydrolyzing); Asparagine synthetase [glutamine-hydrolyzing]; Cell cycle control protein TS11; Glutamine-dependent asparagine synthetase; TS11; TS11 cell cycle control protein Specification REACTIVITY: H M R SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG