CST, 19477S, Sall4 (6E3) Mouse Monoclonal Antibody

Monoclonal Antibody for studying SALL4. Validated for Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Sall4 (6E3) Mouse Monoclonal Antibody (CST #19477) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:100 - 1:400 Storage Supplied as liquid tissue culture supernatant containing sodium azide as a preservative. Stable for 12 months when stored at 4°C. Do not aliquot the antibody. Protocol Available protocols: Immunohistochemistry (Paraffin) Specificity / Sensitivity Sall4 (6E3) Mouse Monoclonal Antibody recognizes endogenous levels of Sall4 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a partial recombinant protein with GST tag corresponding to human Sall4 protein. Background Members of the gene family encode putative zinc finger transcription factors highly expressed during development (1). Sall4 is expressed very early in development with other pluripotency regulators, such as Oct-4 and Nanog (2). Recent studies suggest Sall4 works as a master regulator that controls its own expression and the expression of Oct-4 in a transcriptional regulation feedback loop governing stem cell pluripotency and stem cell fate (2,3). Immunohistochemical studies indicate that Sall4 is a sensitive and specific diagnostic marker for primary germ cell tumors and yolk sac tumors (4,5). Research studies have shown that Sall4 is constitutively expressed in acute myeloid leukemia (AML) and is a probable effector of the Wnt/β-catenin signaling pathway in this disease (6). In addition, mutations in have been implicated in human malformation syndromes, including Duane-radial ray syndrome (Okihiro syndrome) and Acro-renal-ocular syndrome (7). Alternate Names dJ1112F19.1; DRRS; HSAL4; MGC133050; sal-like 4 (Drosophila); Sal-like protein 4; SALL4; spalt like transcription factor 4; Zinc finger protein 797; Zinc finger protein SALL4; ZNF797 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Mouse IgG1