CST, 19558SF, PTPRZ1 (E7C8Z) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying PTPRZ1. Validated for Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, PTPRZ1 (E7C8Z) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #19558) is ready to ship. Product Usage Information This product is the carrier free version of product #15641. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 15641 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity PTPRZ1 (E7C8Z) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total PTPRZ1 protein. Non-specific staining was observed in mouse kidney tubules by immunohistochemistry. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human PTPRZ1 protein. Background PTPRZ1, also known as protein tyrosine phosphatase receptor zeta, is a highly glycosylated membrane protein that is expressed abundantly in normal brain, in a spatially restricted pattern. The functions of PTPRZ1 in neural tissue are thought to be diverse, including development, neural development, neural myelination, and memory formation (1). PTPRZ1 can be found in 2 forms, a membrane-associated glycosylated form and a non-glycosylated, secreted form. In patients with glioma, the soluble form is found to be unregulated in cerebrospinal fluid (2). It is for this and other reports, that PTPRZ1 is a target of pharmaceutical interest. PTPRZ1 has also been reported to be upregulated. Interestingly, increased expression of both membrane bound and soluble PTPRZ1 has also been found in various cancer types, including breast, lung, cervical, hepatocellular carcinoma, and renal tumors (3). In breast cancer, the distribution between localizations of membrane and soluble forms has been reported to change with cancer development, progression, and metastasis. Alternate Names HPTPZ; HPTPzeta; HTPZP2; phosphacan; protein tyrosine phosphatase receptor type Z1; protein tyrosine phosphatase, receptor-type, Z polypeptide 1; protein tyrosine phosphatase, receptor-type, zeta polypeptide 1; Protein-tyrosine phosphatase receptor type Z polypeptide 1; Protein-tyrosine phosphatase receptor type Z polypeptide 2; PTP-ZETA; PTP18; PTPRZ; PTPRZ1; PTPRZ2; PTPZ; R-PTP-zeta; R-PTP-zeta-2; receptor-type tyrosine phosphatase beta/zeta; Receptor-type tyrosine-protein phosphatase zeta; RPTPB; RPTPbeta Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG