CST, 19689S, Cystathionine gamma-Lyase (D1N1D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CTH. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Cystathionine gamma-Lyase (D1N1D) Rabbit Monoclonal Antibody (CST #19689) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Cystathionine gamma-Lyase (D1N1D) Rabbit Monoclonal Antibody recognizes endogenous levels of total cystathionine γ-lyase protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro298 of human cystathionine γ-lyase protein. Background Cystathionine γ-lyase (CGL) is an enzyme in the transsulfuration pathway, a route in the metabolism of sulfur-containing amino acids (1). This enzyme regulates local vasodilation and blood pressure by generating hydrogen sulfide (H S) as a physiological signaling molecule (2). A rodent model of sleep apnea showed that H S production by cystathionine γ-lyase in the carotid body triggers hypertension in rodents during intermittent hypoxia, suggesting that inhibition of this enzyme may prevent the hypertension associated with sleep apnea (3). In addition, dietary restriction of sulfur-containing amino acids upregulates hepatic cystathionine γ-lyase expression in mice, leading to elevated production of H S and protection from hepatic ischemia perfusion injury, indicating that this enzyme is critical for the benefits of dietary restriction (4). Alternate Names CGL; CTH; cystathionase (cystathionine gamma-lyase); Cystathionine gamma-lyase; cysteine desulfhydrase; Cysteine-protein sulfhydrase; Gamma-cystathionase; homoserine deaminase; homoserine dehydratase; MGC9471 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 44 Source/Isotype: Rabbit IgG