CST, 20035S, DIS3 Antibody

Polyclonal Antibody for studying DIS3. Validated for Western Blotting. Highly specific and rigorously validated in-house, DIS3 Antibody (CST #20035) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity DIS3 Antibody recognizes endogenous levels of total DIS3 protein. This antibody detects an 85 kDa band of unknown origin. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val928 of human DIS3 protein. Antibodies are purified by peptide affinity chromatography. Background DIS3, also known as RRP44, is a conserved protein responsible for the core catalytic activities of the exosome complex, which degrades RNA (1-3). With two catalytic sites, DIS3 possesses both endoribonucleolytic and 3'-to-5' exoribonucleolytic activity (2,4). It plays very diverse roles in RNA metabolism, including control of aberrant pre-mRNA turnover, gene expression, and RNA processing (5-9). DIS3 also plays a role in mitotic progression by helping form kinetochores (9,10). Additionally, DIS3 is implicated in antibody diversity by helping the exosome target activation-induced cytidine deaminase to the DNA strands in B cells. DIS3 and other exosome components are upregulated during V(D)J recombination and B cells in knockout mice are unable to develop past the pro-B cell stage (11,12). Mutations in the gene have been well described in multiple myeloma (13,14). Alternate Names 2810028N01Rik; bA555G22.1; DIS3; DIS3 exosome endoribonuclease and 3'-5' exoribonuclease; DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease; DIS3 mitotic control homolog; DIS3 mitotic control homolog (S. cerevisiae); dis3p; DKFZp667L1817; EXOSC11; Exosome complex exonuclease RRP44; exosome component 11; FLJ10484; KIAA1008; MGC33035; mitotic control protein dis3 homolog; Protein DIS3 homolog; Ribosomal RNA-processing protein 44; RP11-342J4.3; RRP44 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 120 SOURCE: Rabbit