CST, 20049SF, PML (E6S9L) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying PML human. Validated for Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, PML (E6S9L) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #20049) is ready to ship. Product Usage Information This product is the carrier free version of product #69789. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 69789 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity PML (E6S9L) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total PML protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human PML protein. Background Promyelocytic leukemia protein (PML) functions via its association with PML bodies in the nucleus (1). PML bodies are dynamic protein aggregates that are interspersed between chromatin in the nuclei of most mammalian cells. The PML protein acts as a scaffold in PML bodies to recruit other proteins, a process regulated by post-translational modifications, such as sumoylation. PML bodies function to regulate a large number of cellular processes, such as tumor suppression, transcriptional regulation, apoptosis, senescence, DNA damage response, and viral defense (1). The chromosomal translocation t(15;17)(q21;q21) involving the fusion of PML and RARalpha is found in acute promyelocytic leukemia (APL), while a second translocation t(9;15)(p13;q24) involving the fusion of PML and PAX5 was found in acute lymphoblastic leukemia (ALL) (2-6). In addition, PML is frequently inactivated or downregulated in cancer. Alternate Names MYL; PML; PML nuclear body scaffold; PML/RARA fusion; PP8675; Probable transcription factor PML; promyelocytic leukemia; promyelocytic leukemia protein; promyelocytic leukemia, inducer of; Protein PML; RING finger protein 71; RNF71; TRIM19; tripartite motif protein TRIM19; Tripartite motif-containing protein 19 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 48-200 Source/Isotype: Rabbit IgG