CST, 20085S, FcRn (E4D5I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FcRn mouse. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, FcRn (E4D5I) Rabbit Monoclonal Antibody (CST #20085) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity FcRn (E4D5I) Rabbit Monoclonal Antibody recognizes endogenous levels of total FcRn protein. Non-specific staining was observed in mouse stomach by immunohistochemistry. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with mouse FcRn recombinant protein. Background The neonatal Fc receptor, FcRn, is an atypical Fc gamma receptor that bears homology to class I MHC (major histocompatibility complex) and associates with β2-microglobulin (1). FcRn is expressed by the vascular endothelium, several epithelial cell types, and hematopoietic cells including several innate immune cell types and B cells (2). FcRn mediates recycling and transcytosis of IgG, resulting in protection of IgG from catabolism and an extended serum half-life of IgG relative to other antibody isotypes (3). In addition, FcRn transports IgG across the placenta and also mediates uptake of maternal IgG from milk, resulting in enhanced humoral immunity in the fetus and neonates (1,4,5). FcRn also plays an important role in initiation of adaptive immunity by transporting immune complexes to intracellular dendritic cell compartments for antigen processing and presentation to T cells (6-8). In addition to IgG, FcRn also binds albumin and similarly protects it from catabolism, leading to extended half-life and high plasma concentrations (9). Alternate Names Fc receptor, IgG, alpha chain transporter; FCGRN; Fcgrt; FcRn; igG Fc fragment receptor transporter alpha; IgG Fc fragment receptor transporter alpha chain; IgG Fc receptor; IgG receptor FcRn large subunit p51; neonatal Fc receptor; Q61559 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 50-60 Source/Isotype: Rabbit IgG