CST, 2010T, Atg12 Antibody

Polyclonal Antibody for studying ATG12. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Atg12 Antibody (CST #2010) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Atg12 Antibody detects endogenous levels of total free and Atg5 bound Atg12 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Atg12. Antibodies are purified by peptide affinity chromatography. Background Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related ( ) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8). Alternate Names APG12; Apg12 (autophagy, yeast) homolog; APG12 autophagy 12-like; APG12-like; APG12L; ATG12; ATG12 autophagy related 12 homolog; ATG12 autophagy related 12 homolog (S. cerevisiae); autophagy related 12; Autophagy-related protein 12; FBR93; HAPG12; Ubiquitin-like protein ATG12 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 16, 55 SOURCE: Rabbit