CST, 2012S, LDHA Antibody

Polyclonal Antibody for studying LDH-A. Validated for Western Blotting. Highly specific and rigorously validated in-house, LDHA Antibody (CST #2012) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity LDHA Antibody detects endogenous levels of total LDHA protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys14 of human LDHA protein. Antibodies are purified by peptide affinity chromatography. Background Lactate dehydrogenase (LDH) catalyzes the interconversion of pyruvate and NADH to lactate and NAD . When the oxygen supply is too low for mitochondrial ATP production, this reaction recycles NADH generated in glycolysis to NAD , which reenters glycolysis. The major form of LDH found in muscle cells is the A (LDHA) isozyme. The LDHA promoter contains HIF-1α binding sites (1). LDHA expression is induced under hypoxic conditions (2). During intensive and prolonged muscle exercise, lactate accumulates in muscle cells when the supply of oxygen does not meet demand. When oxygen levels return to normal, LDH converts lactate to pyruvate to generate ATP in the mitochondrial electron transport chain. Alternate Names cell proliferation-inducing gene 19 protein; epididymis secretory sperm binding protein Li 133P; GSD11; HEL-S-133P; L-lactate dehydrogenase A chain; lactate dehydrogenase A; lactate dehydrogenase M; LDH muscle subunit; LDH-A; LDH-M; LDH1; LDHA; LDHM; PIG19; proliferation-inducing gene 19; Renal carcinoma antigen NY-REN-59 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 37 SOURCE: Rabbit