CST, 20138SF, HOP (D10E2) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying HOP. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, HOP (D10E2) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #20138) is ready to ship. Product Usage Information This product is the carrier free version of product #5670. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 5670 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity HOP (D10E2) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total HOP protein. This antibody is not known to cross-react with other heat shock proteins. Species Reactivity: Human, Monkey, Bovine Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro391 of human HOP protein. Background HOP, also known as stress-induced phospho protein 1 (STIP), is a co-chaperone to the major heat shock proteins, Hsp70 and Hsp90, and appears in early receptor complexes (1,2). Through mutual binding to both Hsp70 and Hsp90, Hop functions as an adaptor that can integrate Hsp70 and Hsp90 interactions (3,4). HOP is an abundant and highly conserved protein which is composed of three tetratricopeptide repeat (TPR) domains (TPR1, TPR2a and TPR2b) and two DP repeat domains (DP1 and DP2), whose function has not been fully resolved (5). Alternate Names epididymis secretory sperm binding protein Li 94n; HEL-S-94n; Hop; Hsc70/Hsp90-organizing protein; Hsp70/Hsp90-organizing protein; IEF-SSP-3521; NY-REN-11 antigen; P60; Renal carcinoma antigen NY-REN-11; STI1; STI1L; STIP1; stress induced phosphoprotein 1; Stress-induced-phosphoprotein 1; stress-induced-phosphoprotein 1 (Hsp70/Hsp90-organizing protein); Transformation-sensitive protein IEF SSP 3521 Specification REACTIVITY: H Mk B SENSITIVITY: Endogenous MW (kDa): 64 Source/Isotype: Rabbit IgG