CST, 2014S, ZPR1 (LG1) Mouse Monoclonal Antibody

Monoclonal Antibody for studying ZPR1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, ZPR1 (LG1) Mouse Monoclonal Antibody (CST #2014) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ZPR1 (LG1) Mouse Monoclonal Antibody detects endogenous levels of total ZPR1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with purified recombinant human ZPR1. Background The zinc finger protein ZPR1 (ZNF259) binds to epidermal growth factor receptor (EGFR) and is localized to both cytoplasm and nucleus. The zinc fingers found in ZPR1 and the tyrosine kinase domain of EGFR mediate the interaction between ZPR1 and the receptor (1). ZPR1 translocates from the cytoplasm to nucleus following mitogen (i.e. EGF) stimulation (2,3). ZPR1 also interacts with translation elongation factor eEF1A following EGF treatment (3). The interaction between the zinc finger protein and elongation factor is important for cell proliferation. Cells lacking ZPR1 exhibit abnormal nucleolar function, suggesting that ZPR1 is required for cell viability and nucleolar function in dividing cells (3). ZPR1 knockout mice exhibit significant neurodegeneration, and reduced or altered expression of ZPR1 may contribute to spinal muscular atrophy, a disorder characterized by degeneration of spinal cord neurons (4). Alternate Names MGC110983; Zinc finger protein 259; Zinc finger protein ZPR1; ZNF259; ZPR1; ZPR1 zinc finger Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 51 Source/Isotype: Mouse IgG1