CST, 20172S, Phospho-LAT (Tyr220) (E3S5L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LAT (Tyr220) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-LAT (Tyr220) (E3S5L) Rabbit Monoclonal Antibody (CST #20172) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 84593 . Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-LAT (Tyr220) (E3S5L) Rabbit Monoclonal Antibody recognizes endogenous levels of LAT protein when phosphorylated at Tyr220. This antibody shows cross-reactivity with phosphorylated EGFR, and detects a 70 kDa band of unknown origin in some treated cell lines. Tyr220 of LAT, long isoform corresponds to Tyr191 of LAT, short isoform. Weak cross-reactivity with phosphorylated tyrosines 171 and 255 of LAT has been observed. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr220 of human LAT protein. Background LAT, a transmembrane adaptor protein expressed in T, NK, and mast cells, is an important mediator for T cell receptor (TCR) signaling (1). Upon TCR engagement, activated Zap-70 phosphorylates LAT at multiple conserved tyrosine residues within SH2 binding motifs, exposing these motifs as the docking sites for downstream signaling targets (2,3). The phosphorylation of LAT at Tyr171 and Tyr220 enables the binding of Grb2, Gads/SLP-76, PLCγ1, and PI3 kinase through their SH2 domain and translocates them to the membrane. This process eventually leads to activation of the corresponding signaling pathways (1-4). Alternate Names 36 kDa phospho-tyrosine adapter protein; 36 kDa phospho-tyrosine adaptor protein; IMD52; LAT; LAT1; linker for activation of T cells; linker for activation of T cells, transmembrane adaptor; Linker for activation of T-cells family member 1; p36-38; pp36 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 40 Source/Isotype: Rabbit IgG