CST, 20173T, Melanotransferrin (F3W8H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MFI2. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Melanotransferrin (F3W8H) Rabbit Monoclonal Antibody (CST #20173) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Melanotransferrin (F3W8H) Rabbit Monoclonal Antibody recognizes endogenous levels of total melanotransferrin protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly668 of human melanotransferrin protein. Background Melanotransferrin, also known as MTF, MFI2, CD228, or p97, is a membrane-bound glycoprotein that is commonly associated with melanoma (1). Melanotransferrin shares structural similarity and iron-binding properties with the protein transferrin, although it does not appear to be involved with iron transport (2-4). Melanotransferrin is expressed at low levels in normal tissue but has elevated expression in melanoma (5,6). The exact biological function of melanotransferrin in melanoma is not yet fully understood, but it is believed to play a role in cell proliferation and invasion (7). Upregulation of melanotransferrin has also been observed in other forms of cancer, such as lung, colorectal, gastric, and oral squamous cell carcinoma (OSCC) (8-11). Alternate Names antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5; CD228; FLJ38863; MAP97; Melanoma-associated antigen p97; melanoma-associated antigen p97, isoform 2; Melanotransferrin; MELTF; membrane-bound transferrin-like protein; MFI2; MGC4856; MTf; MTF1; TRFM Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 97 Source/Isotype: Rabbit IgG