CST, 2019T, FoxD3 (D20A9) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FoxD3. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, FoxD3 (D20A9) Rabbit Monoclonal Antibody (CST #2019) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity FoxD3 (D20A9) Rabbit Monoclonal Antibody detects transfected levels of total FoxD3 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues at the C-terminus of human FoxD3. Background FoxD3 is a member of the Forkhead Box family and is characterized by a winged-helix DNA-binding structure and the important role it plays in embryonic development (1). This transcriptional regulator is required for the maintenance of pluripotency in the pre-implantation and peri-implantation stages of mouse embryonic development (2) and is also required for trophoblast formation (3). FoxD3 is required for the maintenance of the mammalian neural crest; FoxD3 mouse embryos fail around the time of implantation with loss of neural crest-derived structures (4). FoxD3 also forms a regulatory network with Oct-4 and NANOG to maintain the pluripotency of ES cells (5,6). Alternate Names AIS1; forkhead box D3; Forkhead box protein D3; FOXD3; Genesis; HFH2; HNF3/FH transcription factor genesis; VAMAS2 Specification REACTIVITY: H SENSITIVITY: Transfected Only MW (kDa): 60 Source/Isotype: Rabbit IgG