CST, 20218S, GluD2/GRID2 (E4I9V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GluR-delta2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, GluD2/GRID2 (E4I9V) Rabbit Monoclonal Antibody (CST #20218) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity GluD2/GRID2 (E4I9V) Rabbit Monoclonal Antibody recognizes endogenous levels of total GluD2/GRID2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human GluD2/GRID2 protein. Background Glutamate dehydrogenase 1 and 2 (GluD1 and GluD2, respectively), also known as glutamate receptor ionotropic, delta-1 and delta-2 (GRID1 and GRID2, respectively), are part of the delta family of ionotropic glutamate receptors (iGluRs). Ionotropic receptors, typically cation-permeable ligand-gated ion channels, mediate most excitatory transmission in the brain (1). iGluR classes are defined based on sequence identity and pharmacological properties and include α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors, kainate receptors, and N-methyl-D-aspartate (NMDA) receptors (2). Although GluDs were identified based on sequence identity and their structure is related to other iGluRs, GluDs do not bind glutamate and are unlikely to form ion channels like other iGluRs (3). GluD1 and GluD2 share 56% sequence homology. While GluD2 expression is highest in the cerebellum and likely regulates motor function, GluD1 is expressed throughout the forebrain. While GluDs may not function like typical ligand-gated ion channels, they bind to other synaptic proteins and may function to alter subcellular expression of synaptic proteins, including other iGluRs as well as synaptic adhesion proteins. Genetic studies in mice suggest GluDs function in higher-order functions, including learning, memory, and addiction. Alternate Names GluD2; GluR delta-2 subunit; GluR-delta-2; GluR-delta2; GLURD2; glutamate ionotropic receptor delta type subunit 2; Glutamate receptor delta-2 subunit; Glutamate receptor ionotropic, delta-2; glutamate receptor, ionotropic, delta 2; GRID2; MGC117022; MGC117023; MGC117024; SCAR18 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 113 Source/Isotype: Rabbit IgG