CST, 2024T, Hexokinase I (C35C4) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Hexokinase 1. Validated for WB,IP,IHC,IF,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Hexokinase I (C35C4) Rabbit Monoclonal Antibody (CST #2024) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:800 Immunofluorescence (Frozen): 1:800 - 1:1600 Immunofluorescence (Immunocytochemistry): 1:200 Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 75806 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Hexokinase I (C35C4) Rabbit Monoclonal Antibody detects endogenous levels of total hexokinase I protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu108 of human hexokinase I protein. Background Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Four distinct mammalian hexokinase isoforms, designated as hexokinase I, II, III, and IV (glucokinase), have been identified. Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) (1) in order to compensate for the increased energy demands associated with rapid cell growth and proliferation (2,3). Alternate Names brain form hexokinase; glycolytic enzyme; hexokinase; hexokinase 1; Hexokinase 1 isoform HKI variant; hexokinase IR; hexokinase type I; Hexokinase-1; Hexokinase-A; HK; HK I; HK1; HK1-ta; HK1-tb; HK1-tc; HKD; HKI; HMSNR; HXK1; NEDVIBA; RP79 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 102 Source/Isotype: Rabbit IgG