CST, 20256T, APOC2 (F4Q3M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying APOC2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, APOC2 (F4Q3M) Rabbit Monoclonal Antibody (CST #20256) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity APOC2 (F4Q3M) Rabbit Monoclonal Antibody recognizes endogenous levels of total APOC2 protein. This antibody detects a 45 kDa and a 65 kDa protein of unknown identity in some cell lines. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val93 of human APOC2 protein. Background Apolipoprotein C-II (APOC2) is a secreted cofactor that promotes triglyceride hydrolysis by lipoprotein lipase (LPL) (1). Several pathogenic APOC2 variants that lead to APOC2 deficiency and high triglyceride levels have been identified (2,3). There is also data suggesting that APOC2 is upregulated in various forms of cancer and may influence metabolic pathways of cancer cells (4). In acute myeloid leukemia, APOC2 was found to be highly expressed and to promote cancer cell proliferation by interacting with CD36 and upregulating signaling through Lyn and Erk (5). APOC2 was also found to be overexpressed in gastric cancer and to promote cancer cell invasion, migration, and proliferation by upregulation of PI3K, Akt, and mTOR signaling (6). Alternate Names APC2; Apo-CII; ApoC-II; APOC2; Apolipoprotein C-II; Apolipoprotein C2; MGC75082; ProapoC-II; Proapolipoprotein C-II Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 9 Source/Isotype: Rabbit IgG