CST, 2025S, Miwi (D478) Antibody

Polyclonal Antibody for studying Miwi. Validated for Western Blotting. Highly specific and rigorously validated in-house, Miwi (D478) Antibody (CST #2025) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Miwi (D478) Antibody detects endogenous levels of total Miwi protein. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp478 of mouse Miwi. Antibodies are purified by protein A and peptide affinity chromatography. Background The piwi gene was identified as being required for the self-renewal of germline stem cells (1). Piwi homologs are well conserved among various species including , and (1). Both Miwi and Mili proteins are mouse homologs of Piwi and contain a C-terminal Piwi domain (2). Miwi and Mili bind to Piwi-interacting RNAs (piRNAs) in male germ cells and are essential for spermatogenesis in mice (3-5). Alternate Names CT80.1; HIWI; MIWI; PIWI; piwi homolog; piwi like RNA-mediated gene silencing 1; piwi-like 1 (Drosophila); Piwi-like protein 1; PIWIL1; PIWL1 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 90 SOURCE: Rabbit