CST, 20296S, Phospho-Tau (Ser46) Antibody

Polyclonal Antibody for studying Tau iso8 (Ser46) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-Tau (Ser46) Antibody (CST #20296) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-Tau (Ser46) Antibody recognizes endogenous levels of Tau protein only when phosphorylated at Ser46. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser46 of human Tau protein. Antibodies are purified by peptide affinity chromatography. Background Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, glycogen synthase kinase-3 (GSK-3), and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease (AD); these tangles are bundles of paired helical filaments (PHFs) composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3). Phosphorylation of Tau at Ser46 is induced by different kinases, including GSK-3β (4), casein kinase-1 (5), and p38MAPK (6). Tau phosphorylation at Ser46 has been linked to different neurodegenerative diseases, including in PHF-Tau from Alzheimer's disease (4,6), Progressive Supranuclear Palsy (7), and synucleinopathies (6). Alternate Names DDPAC; FLJ31424; FTDP-17; G protein beta1/gamma2 subunit-interacting factor 1; MAPT; MAPTL; MGC138549; microtubule associated protein tau; Microtubule-associated protein tau; MSTD; MTBT1; MTBT2; Neurofibrillary tangle protein; Paired helical filament-tau; PHF-tau; PPND; PPP1R103; protein phosphatase 1, regulatory subunit 103; TAU; Tau iso8 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 50-80 SOURCE: Rabbit