CST, 20459T, FoxM1 (D3F2B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FOXM1. Validated for WB,IP,IHC,ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, FoxM1 (D3F2B) Rabbit Monoclonal Antibody (CST #20459) is ready to ship. Product Usage Information For optimal ChIP results, use 5uL of antibody and 10ug of chromatin (approximately 4 x 10^6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:300 - 1:1200 Chromatin IP: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 40826 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Chromatin IP Specificity / Sensitivity FoxM1 (D3F2B) Rabbit Monoclonal Antibody recognizes endogenous levels of total FoxM1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val699 of human FoxM1 protein. Background Forkhead box M1 (FoxM1) is a forkhead box family transcription factor that regulates a number of genes throughout the cell cycle to help control DNA replication, mitosis, and cell proliferation. FoxM1 expression increases during G1 and S and reaches maximum levels in G2/M (1-3). Nuclear translocation occurs just before entry into G2/M and is associated with FoxM1 phosphorylation (4). Phosphorylation of FoxM1 by MAPK (Ser331, Ser704), Cyclin/Cdk (Ser4, Ser35, Thr600, Thr611, Thr620, Thr627, Ser638), Plk1 (Ser715, Ser724), and Chk2 (Ser376) stabilizes and activates FoxM1 (4-8). Forkhead box M1 is expressed in all embryonic tissues but is restricted to proliferating tissues in adults (9). Research studies show that FoxM1 expression is negatively regulated by p53 (10,11). Upregulation of FoxM1 is associated with many human cancers, including prostate, breast, lung, ovary, colon, pancreas, stomach, bladder, liver, and kidney, and may be associated with p53 mutations in some tumors (11,12). As a result, FoxM1 inhibitors have become a topic of interest for potential cancer therapy (13). Alternate Names FKHL16; forkhead box M1; forkhead box M1-D; Forkhead box protein M1; forkhead-like 16; Forkhead-related protein FKHL16; Forkhead, drosophila, homolog-like 16; FOXM1; FOXM1A; FOXM1B; FOXM1C; Hepatocyte nuclear factor 3 forkhead homolog 11; HFH-11; HFH11; HNF-3; HNF-3/fork-head homolog 11; INS-1; M-phase phosphoprotein 2; MPHOSPH2; MPM-2 reactive phosphoprotein 2; MPP-2; MPP2; PIG29; TGT3; Transcription factor Trident; TRIDENT; WIN; Winged-helix factor from INS-1 cells Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 110 Source/Isotype: Rabbit IgG