CST, 2046S, NGF Antibody

Polyclonal Antibody for studying NGF beta. Validated for Western Blotting. Highly specific and rigorously validated in-house, NGF Antibody (CST #2046) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity NGF Antibody detects recombinant NGF protein. This antibody does not cross-react with other proteins. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr146 of human NGF. Background Nerve growth factor (NGF) is a small, secreted protein and member of the neurotrophin family of growth factors that promote neuronal cell survival and differentiation (1). Producing cells release NGF that bind and activate TrkA high affinity receptors to mediate NGF-driven signaling. NGF also binds to low affinity p75 (NTR) receptors, which belong to the death receptor family (2). Although NGF has been classically described as favoring neuron survival and differentiation, NGF can promote apoptosis in cells that contain p75 (NTR) and lack TrkA. NGF can induce neuron death in a variety of neurodegenerative conditions, including Alzheimer's disease (3). Besides its neurotrophic actions, NGF has an effect on non-neuronal cells and may help mediate inflammation, angiogenesis, and stimulate breast cancer cell growth (4-6). NGF signaling is looking increasingly promising as potential drug targets for diseases. Alternate Names Beta-nerve growth factor; Beta-NGF; HSAN5; MGC161426; MGC161428; nerve growth factor; nerve growth factor (beta polypeptide); nerve growth factor, beta polypeptide; nerve growth factor, beta subunit; NGF; NGFB; pro-nerve growth factor long Specification REACTIVITY: H SENSITIVITY: Recombinant protein MW (kDa): 13 SOURCE: Rabbit