CST, 20488T, Integrin beta 2 (E2Z7F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Integrin Beta2 mouse. Validated for Flow Cytometry (Live). Available in 2 sizes. Highly specific and rigorously validated in-house, Integrin beta 2 (E2Z7F) Rabbit Monoclonal Antibody (CST #20488) is ready to ship. Product Usage Information Flow Cytometry (Live): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Flow Cytometry (Live) Specificity / Sensitivity Integrin beta 2 (E2Z7F) Rabbit Monoclonal Antibody recognizes endogenous levels of total integrin beta 2 protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro101 of mouse integrin beta 2 protein. Background Integrins are transmembrane glycoproteins that form heterodimers consisting of one α and one β subunit. Integrin dimers act as receptors for extracellular matrix proteins and cell-surface ligands. Integrin signaling to (inside-out) and from (outside-in) extracellular molecules regulates multiple cellular processes, such as development, wound healing, immune response, invasion, metastasis, and angiogenesis (1,2). Integrin β2 (CD18) is the β subunit of the leukocyte-specific integrin family. Leukocyte integrins include Integrin β2 (CD18)/αL (CD11a) (LFA-1, lymphocyte function associated antigen 1), Integrin β2 (CD18)/αM (CD11b) (Mac-1), Integrin β2 (CD18)/αX (CD11c), and Integrin β2 (CD18)/αD (CD11d). These integrins bind to immunoglobulin superfamily members, such as ICAM-1 and VCAM-1 on endothelial cells, to mediate firm adhesion and transendothelial migration of leukocytes (3). Integrin β2 (CD18) deficiency results in LAD (leukocyte adhesion deficiency), a disease characterized by impairment of leukocyte recruitment, resulting in an inability to fight infection (4). Alternate Names 2E6; AI528527; Cd18; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; Complement receptor C3 subunit beta; integrin beta 2; Integrin beta-2; ITB2; Itgb2; LAD; LCAMB; Lfa1; lymphocyte function associated antigen 1; Mac-1 beta; macrophage antigen-1 beta; MF17; OTTMUSP00000021575 Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG