CST, 20527S, Phospho-Caveolin-1 (Tyr14) (F2Q7Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying caveolin-1 (Tyr14) phosphate. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Caveolin-1 (Tyr14) (F2Q7Y) Rabbit Monoclonal Antibody (CST #20527) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-Caveolin-1 (Tyr14) (F2Q7Y) Rabbit Monoclonal Antibody recognizes endogenous levels of caveolin-1 protein only when phosphorylated at Tyr14. This antibody cross-reacts with tyrosine phosphorylated EGF, PDGF beta, and HER2/HER3/HER4. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr14 of human caveolin-1 protein. Background The 21-24 kDa integral proteins, caveolins, are the principal structural components of the cholesterol/sphingolipid-enriched plasma membrane microdomain caveolae. Three members of the caveolin family (caveolin-1, -2, and -3) have been identified with different tissue distributions. Caveolins form hetero- and homo-oligomers that interact with cholesterol and other lipids (1). Caveolins are involved in diverse biological functions, including vesicular trafficking, cholesterol homeostasis, cell adhesion, and apoptosis, and are also implicated in neurodegenerative disease (2). Caveolins interact with multiple signaling molecules, such as Gα subunit, tyrosine kinase receptors, PKCs, Src family tyrosine kinases, and eNOS (1,2). It is believed that caveolins serve as scaffolding proteins for the integration of signal transduction. Phosphorylation at Tyr14 is essential for caveolin association with SH2 or PTB domain-containing adaptor proteins, such as GRB7 (3-5). Phosphorylation at Ser80 regulates caveolin binding to the ER membrane and entry into the secretory pathway (6). Alternate Names BSCL3; CAV; CAV1; caveolin 1; caveolin 1, caveolae protein, 22kDa; Caveolin-1; cell growth-inhibiting protein 32; CGL3; LCCNS; MSTP085; PPH3; VIP21 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 23, 25 Source/Isotype: Rabbit IgG