CST, 20617T, B7-H6 (E9Q7L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying B7-H6. Validated for Immunoprecipitation,Flow Cytometry (Fixed/Permeabilized),Flow Cytometry (Live). Available in 2 sizes. Highly specific and rigorously validated in-house, B7-H6 (E9Q7L) Rabbit Monoclonal Antibody (CST #20617) is ready to ship. Product Usage Information Immunoprecipitation: 1:200 Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600 Flow Cytometry (Live): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunoprecipitation, Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity B7-H6 (E9Q7L) Rabbit Monoclonal Antibody recognizes endogenous levels of total B7-H6 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro185 of human B7-H6 protein. Background B7-H6 (NCR3LG1) is a member of the B7 family of proteins that provide costimulatory signals for regulating T cell responses (1-3). B7-H6 is a ligand for the natural killer (NK)-cell-activating receptor NKp30, which triggers anti-tumor NK cell cytotoxicity and cytokine secretion (1). Both forms of B7-H6, cell surface B7-H6 and soluble B7-H6 (sB7-H6), are detected in primary tumors and the tumor microenvironment, but rarely in normal tissues (1-5). Cell surface B7-H6 associates with cell damage, and triggers innate immunity when it binds to NKp30 on NK cells (3,5). Soluble B7-H6 can decrease NKp30 expression on NK cells and induce NK cell dysfunction (4,5). Alternate Names B7 homolog 6; B7-H6; B7H6; DKFZp686I21167; DKFZp686O24166; hypothetical protein DKFZp686O24166; Natural cytotoxicity triggering receptor 3 ligand 1; natural killer cell cytotoxicity receptor 3 ligand 1; NCR3LG1; NR3L1; Putative Ig-like domain-containing protein DKFZp686O24166/DKFZp686I21167; YK047 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG