CST, 2064S, WIF1 Antibody

Polyclonal Antibody for studying WIF1. Validated for Western Blotting. Highly specific and rigorously validated in-house, WIF1 Antibody (CST #2064) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity WIF1 Antibody detects transfected levels of total WIF1 protein. Species Reactivity: Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln166 of human WIF1. Antibodies are purified by peptide affinity chromatography. Background WIF1 (Wnt inhibitory factor 1) is a secreted protein that binds to Wnt proteins and inhibits their activity (1). It contains an N-terminal WIF domain and five EGF-like repeats (2). The WIF1 ortholog in Drosophila, Shifted, is required for Hedgehog stability and diffusion (3,4). It has been reported that WIF1 expression is downregulated in many types of cancers (5-8). Alternate Names WIF-1; WIF1; Wnt inhibitory factor 1 Specification REACTIVITY: M SENSITIVITY: Transfected Only MW (kDa): 42 SOURCE: Rabbit