CST, 2067S, eIF4E (C46H6) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying eIF4E. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, eIF4E (C46H6) Rabbit Monoclonal Antibody (CST #2067) is ready to ship. Product Usage Information For a carrier free (BSA and azide free) version of this product see product # 32042 Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:800 - 1:3200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 32042 Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity eIF4E (C46H6) Rabbit Monoclonal Antibody detects endogenous levels of total eIF4E protein. This antibody does not cross-react with 4EHP protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to human eIF4E protein. Background Eukaryotic initiation factor 4E (eIF4E) binds to the mRNA cap structure to mediate the initiation of translation (1,2). eIF4E interacts with eIF4G, a scaffold protein that promotes assembly of eIF4E and eIF4A into the eIF4F complex (2). eIF4B is thought to assist the eIF4F complex in translation initiation. Upon activation by mitogenic and/or stress stimuli mediated by Erk and p38 MAPK, Mnk1 phosphorylates eIF4E at Ser209 (3,4). Two Erk and p38 MAPK phosphorylation sites in mouse Mnk1 (Thr197 and Thr202) are essential for Mnk1 kinase activity (3). The carboxy-terminal region of eIF4G also contains serum-stimulated phosphorylation sites, including Ser1108, Ser1148, and Ser1192 (5). Phosphorylation at these sites is blocked by the PI3 kinase inhibitor LY294002 and by the FRAP/mTOR inhibitor rapamycin. Alternate Names AUTS19; CBP; eIF-4E; eIF-4F 25 kDa subunit; EIF4E; EIF4E1; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eukaryotic translation initiation factor 4E-like 1; IF4E; MGC111573; mRNA cap-binding protein Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 25 Source/Isotype: Rabbit IgG