CST, 20765S, eIF5A (D8L8Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying eIF5A. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, eIF5A (D8L8Q) Rabbit Monoclonal Antibody (CST #20765) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity eIF5A (D8L8Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total eIF5A protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp104 of human eIF5A protein. Background Eukaryotic initiation factor 5A (eIF5A) is an mRNA-binding protein that is involved in translation elongation and plays an important role in promoting translation of polyproline motifs (1-4). The ( ) and genes encode the two vertebrate eIF5A isoforms. While eIF5A1 is expressed constitutively in all tissues, eIF5A2 is mainly expressed in gonads. eIF5A and eIF5A2 are the only identified proteins that contain the distinctive amino acid hypusine, which is generated posttranslationally from lysine through a highly conserved polyamine metabolism pathway. eIF5A function and hypusine modification are both essential for cell proliferation, as knock down of eIF5A expression or blocking eIF5A hypusine modification suppresses cancer cell proliferation (5-7). Interestingly, eIF5A is an identified component of a tumor suppressor network of the polyamine-hypusine axis. Co-suppression of both eIF5A and adenosylmethionine decarboxylase 1 (AMD1) promotes lymphomagenesis in mice, while heterozygous deletions of the corresponding and genes often occur together in human lymphomas (8). Alternate Names eIF-4D; eIF-5A; eIF-5A-1; eIF-5A1; EIF5A; EIF5A1; eIF5AI; eukaryotic initiation factor 5A; Eukaryotic initiation factor 5A isoform 1; eukaryotic translation initiation factor 5A; Eukaryotic translation initiation factor 5A-1; IF5A1; MGC104255; MGC99547; Rev-binding factor Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 18 Source/Isotype: Rabbit IgG