CST, 2080T, CHIP (C3B6) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CHIP. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, CHIP (C3B6) Rabbit Monoclonal Antibody (CST #2080) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CHIP (C3B6) Rabbit Monoclonal Antibody detects endogenous levels of total CHIP protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues around Leu36 of human CHIP. Background The carboxy terminus of Hsc70-interacting protein (CHIP, STUB1) is a co-chaperone protein and functional E3 ubiquitin ligase that links the polypeptide binding activity of Hsp70 to the ubiquitin proteasome system (1). Cytoplasmic CHIP protein contains three 34-amino acid TPR (tetratricopeptide repeat) domains at its amino terminus and a carboxy-terminal U-box domain. CHIP interacts with the molecular chaperones Hsc70-Hsp70 and Hsp90 through its TPR domain, while E3 ubiquitin ligase activity is confined to the U-box domain (2,3). The binding of CHIP to Hsp70 can stall the folding of Hsp70 client proteins and concomitantly facilitate the U-box dependent ubiquitination of Hsp70-bound substrates (4-6). CHIP appears to play a central role in cell stress protection (7) and is responsible for the degradation of disease-related proteins that include cystic fibrosis transmembrane conductance regulator (4), p53 (8), huntingtin and Ataxin-3 (9), Tau protein (10), and α-synuclein (11). Alternate Names Antigen NY-CO-7; Carboxy terminus of Hsp70-interacting protein; CHIP; CLL-associated antigen KW-8; E3 ubiquitin-protein ligase CHIP; heat shock protein A binding protein 2 (c-terminal); HSPABP2; NY-CO-7; RING-type E3 ubiquitin transferase CHIP; SCA48; SCAR16; SDCCAG7; serologically defined colon cancer antigen 7; STIP1 homology and U box-containing protein 1; STIP1 homology and U-box containing protein 1; STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase; STUB1; UBOX1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 32 Source/Isotype: Rabbit IgG