CST, 2088S, LEDGF (C57G11) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LEDGF/P75. Validated for Western Blotting,Immunohistochemistry (Paraffin),Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, LEDGF (C57G11) Rabbit Monoclonal Antibody (CST #2088) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:50 Flow Cytometry (Fixed/Permeabilized): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 96208 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity LEDGF (C57G11) Rabbit Monoclonal Antibody detects endogenous levels of LEDGF protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with synthetic peptide corresponding to amino acids surrounding Lys461 of human LEDGF. Background The LEDGF (lens epithelium-derived growth factor) gene encodes splice variants P75 and P52 (1). LEDGF (C57G11) Rabbit mAb detects the 75 kDA LEDGF splice form. Both LEDGF variants have transcriptional coactivator functions that may regulate gene transcription and mRNA splicing of a variety of proteins that are essential for cell growth and survival. (2,3). LEDGF has also been identified as a factor that can, in serum free media, stimulate the growth and survival of lens epithelial cells and other cell types such as retinal photoreceptor cells, COS7 cells, skin fibroblasts, and keratinocytes (2). In addition to its function as a transcriptional coactivator, LEDGF has been shown to be involved in the pathogenesis of atopic dermatitis (4) and in the activity of HIV1 integrase. LEDGF binds chromatin throughout the cell cycle, and may act as a chromatin docking factor for HIV integrase and other lentiviral pre-integration complexes. It protects HIV integrase from degradation, and influences the pattern of viral integration into the cell genome (5). Alternate Names CLL-associated antigen KW-7; Dense fine speckles 70 kDa protein; DFS 70; DFS70; LEDGF; Lens epithelium-derived growth factor; MGC74712; p52; p75; PAIP; PC4 and SFRS1 interacting protein 1; PC4 and SFRS1-interacting protein; PSIP1; PSIP2; transcriptional coactivator p52/p75; Transcriptional coactivator p75/p52 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 75 Source/Isotype: Rabbit IgG