CST, 20992S, CD99 (E5N6F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD99. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, CD99 (E5N6F) Rabbit Monoclonal Antibody (CST #20992) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CD99 (E5N6F) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD99 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn71 of human CD99 protein. Background CD99 is a transmembrane protein involved in many cellular functions, including cell adhesion and migration, endocytosis and exocytosis, and intracellular protein trafficking. It is highly expressed in all leukocyte lineages, Sertoli cells, granulosa cells, and pancreatic islet cells (1,2). Due to alternative splicing, there are two isoforms of CD99 that differ at the carboxy terminus. CD99 Type I (CD99wt) is the full-length form containing 185 amino acids and CD99 Type II (CD99sh) contains 161 amino acids. Their expression is differentially regulated and they may have opposite functions in different contexts (3,4). CD99 is expressed in many types of tumors and has been used for differential diagnosis of conventional Ewing sarcoma. It has been actively pursued as a therapeutic target (5,6). On the other hand, CD99 may also play a tumor suppressor role in other tumors, such as Hodgkin's lymphomas, osteosarcomas, and pancreatic tumors (6,7). Alternate Names 12E7; antigen identified by monoclonal 12E7, Y homolog; antigen identified by monoclonal antibodies 12E7, F21 and O13; CD99; CD99 antigen; CD99 molecule; CD99 molecule (Xg blood group); cell surface antigen 12E7; cell surface antigen HBA-71; cell surface antigen O13; E2 antigen; HBA71; MIC2; MIC2 (monoclonal antibody 12E7); MIC2X; MIC2Y; MSK5X; Protein MIC2; surface antigen MIC2; T-cell surface glycoprotein E2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 30 Source/Isotype: Rabbit IgG