CST, 2104S, Grp94 Antibody

Polyclonal Antibody for studying GRp94. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Grp94 Antibody (CST #2104) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Grp94 Antibody detects endogenous levels of total Grp94 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence surrounding Met622 of human Grp94 . Antibodies are purified by protein A and peptide affinity chromatography. Background Secretory proteins are synthesized on polysomes and translocated into the endoplasmic reticulum (ER). Inside ER, these proteins are often modified by disulfide bond formation, amino-linked glycosylation and folding. The ER contains a pool of molecular chaperones, including Grp94, to help ensure correct protein folding. Grp94 is a glucose-regulated protein (1) with sequence homology to Hsp90 (2). In addition to its role in helping to facilitate folding of a number of secretory proteins to their correct conformation (3), studies suggest that Grp94 derived from cancer cells also induces anti-tumor immune responses in mouse tumor models (4, 5). One way in which Grp94 promotes tumor immunogenicity is its ability to bind to and present tumor-derived peptides as antigens (6). Furthermore, Grp94 has also been shown to induce maturation of dendritic cells (7). Taken together, Grp94 functions both as a tumor-specific antigen and as an activator of antigen-presenting cells to elicit an anti-cancer immune response (8). Alternate Names 94 kDa glucose-regulated protein; ECGP; Endoplasmin; endothelial cell (HBMEC) glycoprotein; ENPL; epididymis luminal protein 35; epididymis secretory sperm binding protein Li 125m; glucose regulated protein, 94 kDa; GP96; gp96 homolog; GRP-94; GRP94; heat shock protein 90 beta family member 1; Heat shock protein 90 kDa beta member 1; heat shock protein 90kDa beta (Grp94), member 1; heat shock protein 90kDa beta family member 1; HEL-S-125m; HEL35; HSP90B1; stress-inducible tumor rejection antigen gp96; TRA1; tumor rejection antigen (gp96) 1; Tumor rejection antigen 1; Tumor rejection antigen-1 (gp96) Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 100 SOURCE: Rabbit