CST, 2114S, Rictor (53A2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Rictor. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, Rictor (53A2) Rabbit Monoclonal Antibody (CST #2114) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Rictor (53A2) Rabbit Monoclonal Antibody detects endogenous levels of total Rictor protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln1681 of human Rictor protein. Background Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL, Sin1, and rictor (1). The mTOR-rictor complex phosphorylates Ser473 of Akt/PKB (2). This phosphorylation is essential for full Akt/PKB activation. Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes (3). This complex has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR (4). Alternate Names AVO3; AVO3 homolog; DKFZp686B11164; hAVO3; KIAA1999; mAVO3; MGC39830; PIA; pianissimo; Rapamycin-insensitive companion of mTOR; RICTOR; RICTR; RPTOR independent companion of MTOR complex 2; RPTOR independent companion of MTOR, complex 2; TORC2-specific protein AVO3 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 200 Source/Isotype: Rabbit IgG