CST, 2117T, RhoA (67B9) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RhoA. Validated for WB,WB,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, RhoA (67B9) Rabbit Monoclonal Antibody (CST #2117) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunohistochemistry (Paraffin): 1:800 Immunofluorescence (Immunocytochemistry): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 60382 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity RhoA (67B9) Rabbit Monoclonal Antibody recognizes endogenous levels of total RhoA protein. This antibody does not recognize the related proteins RhoB or RhoC. Species Reactivity: Human, Mouse, Rat, Monkey, Bovine Source / Purification Monoclonal antibody is produced by immunizing animals with synthetic peptides corresponding to residues near the carboxy terminus of human RhoA. Background Rho family small GTPases, including Rho, Rac and cdc42, act as molecular switches, regulating processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. A third level of regulation is provided by the stoichiometric binding of Rho GDP dissociation inhibitor (RhoGDI) (1). RhoA, RhoB and RhoC are highly homologous, but appear to have divergent biological functions. Carboxy-terminal modifications and differences in subcellular localization allow these three proteins to respond to and act on distinct signaling molecules (2,3). Functions of RhoA, the most highly studied of these three, include regulation of actomyosin contractility (4), cytokinesis (5), focal adhesion assembly (6) and cell polarity (7). Alternate Names Aplysia ras-related homolog 12; ARH12; ARHA; EDFAOB; epididymis secretory sperm binding protein; h12; oncogene RHO H12; ras homolog family member A; ras homolog gene family, member A; Rho cDNA clone 12; RHO12; RHOA; RHOH12; small GTP binding protein RhoA; Transforming protein RhoA Specification REACTIVITY: H M R Mk B SENSITIVITY: Endogenous MW (kDa): 21 Source/Isotype: Rabbit IgG