CST, 2121S, Cleaved alpha-Fodrin (Asp1185) Antibody

Polyclonal Antibody for studying Spectrin-alpha2 (Asp1185) cleaved. Validated for Western Blotting. Highly specific and rigorously validated in-house, Cleaved alpha-Fodrin (Asp1185) Antibody (CST #2121) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Cleaved alpha-Fodrin (Asp1185) Antibody detects endogenous levels of the large fragment of alpha-fodrin resulting from cleavage at aspartic acid 1185. This antibody does not recognize full length alpha-fodrin. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues surrounding Asp1185 in human alpha-fodrin. Antibodies are purified by protein A and peptide affinity chromatography. Background Fodrin (also named nonerythroid spectrin) is a universally expressed membrane-associated cytoskeletal protein consisting of alpha- and beta-subunits (1). This protein is important for maintaining normal membrane structure and supporting cell surface protein function (1). Alpha-fodrin is one of the primary targets cleaved by caspases during apoptosis. The full length 240 kDa protein can be cleaved at several sites within its sequence by activated caspases to yield amino-terminal 150 kDa, carboxy-terminal 120 kDa and 35 kDa major products (2-5). Cleavage of alpha-fodrin leads to membrane malfunction and cell shrinkage. Alternate Names (ALPHA)II-SPECTRIN; alpha-fodrin; Alpha-II spectrin; EIEE5; epididymis secretory sperm binding protein; FLJ44613; Fodrin alpha chain; NEAS; non-erythrocytic spectrin alpha; Spectrin alpha chain, brain; Spectrin alpha chain, non-erythrocytic 1; spectrin alpha, non-erythrocytic 1; spectrin, alpha, non-erythrocytic 1 (alpha-fodrin); Spectrin, non-erythroid alpha chain; Spectrin, non-erythroid alpha subunit; SPTA2; SPTAN1; SPTN1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 150 SOURCE: Rabbit