CST, 2129S, Phospho-Rev-erb alpha (Ser55/59) Antibody

Polyclonal Antibody for studying Rev-erbα (Ser55/Ser59) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-Rev-erb alpha (Ser55/59) Antibody (CST #2129) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-Rev-erb alpha (Ser55/59) Antibody detects transfected levels of Rev-erbα protein when phosphorylated at Ser55/59. This antibody does not cross-react with other nuclear receptor proteins, including Rev-erbβ. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser55 and Ser59 of human Rev-erbα. Antibodies are purified by protein A and peptide affinity chromatography. Background Reverse orientation c-erbA gene α (Rev-erbα, EAR-1, or NR1D1) is a widely expressed member of the orphan nuclear receptor family of proteins (1). Rev-erbα is highly expressed in adipose tissue, skeletal muscle, brain and liver, and regulates cellular proliferation and differentiation. Expression increases during differentiation in adipocytes and ectopic expression of Rev-erbα potentiates the adipocyte differentiation of 3T3-L1 cells (2). In addition, expression oscillates with circadian rhythm in liver cells and Rev-erbα regulates expression of BMAL1, ApoA-I and ApoC-III, all key regulators of circadian rhythm (3-7). Phosphorylation of Rev-erbα Ser55 and Ser59 by GSK-3β appears to stabilize Rev-erbα protein levels and is important for synchronizing and maintaining the circadian clock (8). Rev-erbα also regulates inflammation by targeting the NF-κB responsive genes IL-6 and COX-2 (9). Rev-erbα lacks the activation function 2 domain required for ligand-dependent activation of transcription by other members of the nuclear receptor family; thus it behaves as a constitutive repressor protein, recruiting the nuclear receptor co-repressor (N-CoR)/HDAC3 complex to target genes to repress transcription (10). Alternate Names EAR-1; EAR1; HREV; NR1D1; nuclear receptor Rev-ErbA-alpha; Nuclear receptor subfamily 1 group D member 1; nuclear receptor subfamily 1, group D, member 1; Rev-erbA-alpha; Rev-ErbAalpha; REVERBA; REVERBalpha; THRA1; THRAL; thyroid hormone receptor, alpha-like; V-erbA-related protein 1 Specification REACTIVITY: H SENSITIVITY: Transfected Only MW (kDa): 80 SOURCE: Rabbit