CST, 2134S, DJ-1 Antibody

Polyclonal Antibody for studying DJ-1. Validated for Western Blotting. Highly specific and rigorously validated in-house, DJ-1 Antibody (CST #2134) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity DJ-1 Antibody detects endogenous levels of total DJ-1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding amino acid 150 of human DJ-1. Antibodies are purified by protein A and peptide affinity chromatography. Background Parkinson's disease (PD) is characterized by the presence of Lewy bodies (intracellular inclusions) and by the loss of dopaminergic neurons. Research studies have shown that mutations in α-synuclein, Parkin, and DJ-1 are linked to PD (1). α-synuclein is a major component of the aggregates found in Lewy bodies. Parkin is involved in protein degradation through the ubiquitin-proteasome pathway, and investigators have shown that mutations in Parkin cause early onset of PD (1). Loss-of-function mutations in DJ-1 cause early onset of PD, but DJ-1 is associated with multiple functions: it cooperates with Ras to increase cell transformation, it positively regulates transcription of the androgen receptor, and it may function as an indicator of oxidative stress (2-5). Dopamine D2 receptor-mediated functions are greatly impaired in DJ-1 (-/-) mice, resulting in reduced long-term depression (6). Alternate Names DJ-1; DJ1; epididymis secretory sperm binding protein Li 67p; FLJ27376; FLJ34360; FLJ92274; GATD2; HEL-S-67p; Maillard deglycase; Oncogene DJ1; PARK7; Parkinson disease (autosomal recessive, early onset) 7; Parkinson disease protein 7; parkinson protein 7; Parkinsonism associated deglycase; Parkinsonism-associated deglycase; protein deglycase DJ-1; Protein DJ-1; Protein/nucleic acid deglycase DJ-1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 22 SOURCE: Rabbit