CST, 2135S, CD2AP Antibody

Polyclonal Antibody for studying CD2AP. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, CD2AP Antibody (CST #2135) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CD2AP Antibody detects endogenous levels of total CD2AP protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids near the carboxy terminus of human CD2AP. Antibodies are purified by protein A and peptide affinity chromatography. Background CD2AP is a scaffolding protein that is thought to link membrane proteins to the cytoskeleton (1-3). It plays a role in formation of tight junctions in specialized cell types such as the slit diaphragm in the kidney glomerulus (4). CD2AP is also involved in the immunological synapse between CD2 expressing T cells and antigen presenting cells (5). It has been shown that interaction between CD2AP and other cytoskeletal proteins may regulate the endocytosis of EGFR (3). Alternate Names Adapter protein CMS; Cas ligand with multiple SH3 domains; Cas ligand with multiple Src homology 3 (SH3) domains; CD2 associated protein; CD2-associated protein; CD2AP; CMS; DKFZp586H0519 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 80 SOURCE: Rabbit