CST, 2143S, Rab5A Antibody

Polyclonal Antibody for studying Rab5. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Rab5A Antibody (CST #2143) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:150 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Rab5A Antibody detects endogenous levels of total Rab5A protein. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly190 of human Rab5A protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Rab5 is a member of the Ras superfamily of small Rab GTPases. Rab5 is localized at the plasma membrane and early endosomes and functions as a key regulator of vesicular trafficking during early endocytosis (1). The conformational change between Rab5 GTP/GDP states is essential for its biological function as a rate limiting regulator at multiple steps during endocytosis (1,2). Rab5 exerts its function by interacting with several Rab5-specific effectors (1-3). These proteins form complexes with Rab5 on a specialized Rab domain of the endosome and promote recycling of Rab5-cargo targets between endosome and the plasma membrane. Alternate Names RAB5; RAB5A; RAB5A, member RAS oncogene family; RAS-associated protein RAB5A; Ras-related protein Rab-5A Specification REACTIVITY: H M R Hm Mk SENSITIVITY: Endogenous MW (kDa): 25 SOURCE: Rabbit