CST, 2146S, beta-Tubulin Antibody

Polyclonal Antibody for studying tubulin beta-5. Validated for WB,IHC,IF,F. Highly specific and rigorously validated in-house, beta-Tubulin Antibody (CST #2146) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:75 - 1:300 Immunofluorescence (Immunocytochemistry): 1:50 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity The beta-Tubulin Antibody detects endogenous levels of total β-tubulin protein, and does not cross-react with recombinant α-tubulin. Species Reactivity: Human, Mouse, Rat, Monkey, Zebrafish, Bovine Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human β-tubulin. Antibodies are purified by protein A and peptide affinity chromatography. Background The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1). Alternate Names beta 5-tubulin; beta Ib tubulin; beta-4 tubulin; CDCBM6; CSCSC1; epididymis secretory sperm binding protein; M40; MGC117247; MGC16435; OK/SW-cl.56; TBB5; TUBB; TUBB1; TUBB5; Tubulin beta chain; tubulin beta class I; tubulin beta polypeptide; tubulin beta-1 chain; Tubulin beta-5 chain; tubulin, beta; tubulin, beta polypeptide Specification REACTIVITY: H M R Mk Z B SENSITIVITY: Endogenous MW (kDa): 55 SOURCE: Rabbit