CST, 2152S, WSTF Antibody

Polyclonal Antibody for studying WSTF. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, WSTF Antibody (CST #2152) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity WSTF Antibody detects endogenous levels of total WSTF protein. This antibody does not cross-react with other related transcription factors. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human WSTF (WBSCR9). Antibodies are purified by protein A and peptide affinity chromatography. Background The human WSTF gene is located within the common Williams Syndrome (WS) deletion area at chromosome 7q11.23. Several WSTF gene products have been detected with little difference in length of polypeptides (1-3). Functional motifs identified by sequence-homology searches include a PHD-type zinc finger motif followed by a bromodomain. Both motifs are found in many transcription factors, suggesting that WSTF may function as a transcription factor. A Drosophila gene (acf1) was cloned, which encodes two forms of Acf1 proteins with molecular weight 170 kDa and 185 kDa, respectively (4). It was demonstrated that Acf1 is structurally related to the human WSTF gene. Acf1 forms a complex with another protein, ISWI, and functions in the ATP-dependent catalysis of chromatin assembly (4). Alternate Names BAZ1B; bromodomain adjacent to zinc finger domain 1B; Bromodomain adjacent to zinc finger domain protein 1B; bromodomain adjacent to zinc finger domain, 1B; hWALp2; transcription factor WSTF; Tyrosine-protein kinase BAZ1B; WBSC10; WBSCR10; WBSCR9; Williams syndrome transcription factor; Williams-Beuren syndrome chromosomal region 10 protein; Williams-Beuren syndrome chromosomal region 9 protein; Williams-Beuren syndrome chromosome region 10; Williams-Beuren syndrome chromosome region 9; WSTF Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 170, 185 SOURCE: Rabbit