CST, 2154S, Musashi Antibody

Polyclonal Antibody for studying Musashi-1. Validated for Western Blotting. Highly specific and rigorously validated in-house, Musashi Antibody (CST #2154) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Musashi Antibody detects endogenous levels of total Musashi 1 and 2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala100 of human Musashi-1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Musashi-1 and Musashi-2 are RNA-binding proteins which play a role in asymmetric cell division of ectodermal precursor cells by regulating the translation of target mRNA. Both family members augment Notch signaling and repress the translation of m-Numb, a protein that positively modulates differentiation of neural stem cells into neurons. Thus, Musashi contributes to the maintenance of neural stem cells (1). While Musashi-1 is frequently used as a marker for proliferating neural precursor cells, it is also expressed in epithelial stem cells including intestinal and mammary gland stem cells (2-4). Alternate Names MSI1; MSI1H; musashi 1; musashi homolog 1 (Drosophila); musashi RNA binding protein 1; Musashi-1; musashi1; RNA-binding protein Musashi homolog 1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 35 SOURCE: Rabbit