CST, 2157S, MBTPS2 Antibody

Polyclonal Antibody for studying MBTPS2. Validated for Western Blotting. Highly specific and rigorously validated in-house, MBTPS2 Antibody (CST #2157) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity MBTPS2 Antibody detects endogenous levels of total MBTPS2 protein. Species Reactivity: Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val322 of human MBTPS2 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Membrane-bound transcription factor protease site 2 (MBTPS2), also known as site-2 protease (S2P), is a zinc metalloprotease in the Golgi membrane (1,2,3). It regulates cholesterol metabolism (1,2) and unfolded protein response (UPR) (3,4). When cells are deprived of cholesterol, sterol regulatory element-binding proteins (SREBPs) move from the endoplasmic reticulum (ER) to the Golgi apparatus and are cleaved by site-1 protease (S1P) (5,6) and site-2 protease (1,6) sequentially to release the active amino-terminal domains. These amino-terminal domains of SREBPs then translocate into the nucleus to induce expression of genes for cholesterol biosynthesis. During UPR, activating transcription factor 6 (ATF6) transports from ER to Golgi apparatus and is cleaved by S1P and S2P to release a cytosolic fragment. This cytosolic fragment relocates to the nucleus and activates the UPR gene expression (7). Alternate Names BRESEK; Endopeptidase S2P; FLJ32174; IFAP; keratosis follicularis spinulosa decalvans; KFSD; KFSDX; MBTP2; MBTPS2; membrane bound transcription factor peptidase, site 2; membrane-bound transcription factor peptidase, site 2; membrane-bound transcription factor protease, site 2; Membrane-bound transcription factor site-2 protease; OI19; OLMSX; S2P; site-2 protease; SREBPs intramembrane protease; Sterol regulatory element-binding proteins intramembrane protease Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 57 SOURCE: Rabbit