CST, 2171S, Alix (3A9) Mouse Monoclonal Antibody

Monoclonal Antibody for studying Alix. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Alix (3A9) Mouse Monoclonal Antibody (CST #2171) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Alix (3A9) Mouse Monoclonal Antibody detects endogenous levels of total Alix protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with full-length recombinant human Alix protein. Background Alix, a phylogenetically conserved cytosolic scaffold protein, contains an N-terminal Bro1 domain, a coiled-coil region and a C-terminal proline-rich domain (1,2). Originally identified as an ALG-2 (apoptosis-linked gene 2)-interacting protein involved in programmed cell death (3,4), Alix also regulates many other cellular processes, such as endocytic membrane trafficking and cell adhesion through interactions with ESCRT (endosomal sorting complex required for transport) proteins, endophilins, and CIN85 (Cbl-interacting protein of 85 kDa) (5,6). Alternate Names AIP1; ALG-2 interacting protein 1; ALG-2 interacting protein X; ALG-2-interacting protein 1; ALG-2-interacting protein X; ALIX; apoptosis-linked gene 2-interacting protein X; dopamine receptor interacting protein 4; DRIP4; Hp95; KIAA1375; MGC17003; PDC6I; PDCD6-interacting protein; PDCD6IP; programmed cell death 6 interacting protein; Programmed cell death 6-interacting protein Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 95 Source/Isotype: Mouse IgG1