CST, 2175S, Cyclophilin A Antibody

Polyclonal Antibody for studying PPIA. Validated for Western Blotting. Highly specific and rigorously validated in-house, Cyclophilin A Antibody (CST #2175) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Cyclophilin A Antibody detects endogenous levels of total Cyclophilin A protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of Cyclophilin A. Antibodies are purified by protein A and peptide affinity chromatography. Background Cyclophilins are a highly conserved family of peptidylprolyl - -isomerases (PPIA) that are targets of the immunosuppressant drug cyclosporin A (CsA) (1,2). The complex of cyclophilin and CsA can bind to and inhibit calcineurin which leads to inhibition of the transcription factor NFAT and decreased production of cytokines (3,4). As isomerases, cyclophilins have been proposed to aid in protein folding. Cyclophilin A can bind to the p55 Gag protein of HIV and appears necessary for HIV infection (5,6). There is also some evidence that cyclophilins have nuclease activity and play a role in apoptosis (7). Alternate Names Cyclophilin A; Cyclosporin A-binding protein; CYPA; CYPH; epididymis secretory sperm binding protein Li 69p; HEL-S-69p; MGC117158; MGC12404; MGC23397; Peptidyl-prolyl cis-trans isomerase A; Peptidyl-prolyl cis-trans isomerase A, N-terminally processed; peptidylprolyl isomerase A; peptidylprolyl isomerase A (cyclophilin A); PPIA; PPIase A; Rotamase A; T cell cyclophilin Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 18 SOURCE: Rabbit