CST, 2176S, HtrA2 Antibody

Polyclonal Antibody for studying HtrA2/Omi. Validated for Western Blotting. Highly specific and rigorously validated in-house, HtrA2 Antibody (CST #2176) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity HtrA2 Antibody detects endogenous levels of the mature form of HtrA2. This antibody is expected to also recognize the unprocessed form of HtrA2. The unprocessed form is detected in cells overexpressing HtrA2. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe341 of HtrA2. Antibodies are purified by protein A and peptide affinity chromatography. Background High temperature requirement protein A2 (HtrA2)/Omi is a serine protease with homology to the HtrA protein (DegP) and is thought to be involved in apoptosis and stress-induced degradation of misfolded proteins (1). While HtrA2 was orignally identified to be present in either the nucleus (1) or endoplasmic reticulum (2), subsequent studies have shown that it localizes in mitochondria and is released during apoptosis (3-7). HtrA2 is produced as a 50 kDa zymogen that is cleaved to generate a 36 kDa mature protein that exposes an amino terminal motif (AVPS) resembling that of the IAP inhibitor Smac/Diablo (3-7). Like Smac, interaction between HtrA2 and IAP family members, such as XIAP, antagonizes their inhibition of caspase activity and protection from apoptosis (3-7). Interestingly, HtrA2 knock-out mice did not show signs of reduced apoptosis, but rather had a loss of neurons in the striatum and a Parkinson's-like phenotype, suggesting that HtrA2 might have a neuroprotective function (8-10). This activity is associated with the protease activity of HtrA2 (8). Furthermore, research studies have shown that loss of function mutations in the HtrA2 gene are associated with Parkinson's disease (11). Alternate Names epididymis secretory sperm binding protein; High temperature requirement protein A2; HtrA serine peptidase 2; HtrA-like serine protease; HTRA2; MGCA8; OMI; Omi stress-regulated endoprotease; PARK13; protease, serine, 25; PRSS25; Serine protease 25; Serine protease HTRA2, mitochondrial; Serine proteinase OMI Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 36 SOURCE: Rabbit