CST, 2177S, Ataxin-1 Antibody

Polyclonal Antibody for studying ataxin-1. Validated for Western Blotting. Highly specific and rigorously validated in-house, Ataxin-1 Antibody (CST #2177) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Ataxin-1 Antibody detects endogenous levels of total ataxin-1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human ataxin-1. Antibodies are purified by protein A and peptide affinity chromatography. Background Spinocerebellar ataxia type 1 (SCA1), an autosomal dominant neurodegenerative disorder, is characterized by slurred speech, loss of limb coordination, and gait abnormalities resulting from the degeneration of cerebellar Purkinje cells and a subset of brainstem neurons (1). Individuals with SCA1 have a highly polymorphic CAG repeat expansion encoding a polyglutamine tract in ataxin-1 (2). Akt phosphorylates ataxin-1 at Ser776, which regulates an association with 14-3-3. This interaction increases ataxin-1 stabilization and accumulation resulting in enhanced neurodegeneration (3). In addition, HSP70 controls the effect that phosphorylation has on ataxin-1 stability (4). Alternate Names alternative ataxin1; ataxin 1; Ataxin-1; ATX1; ATXN1; D6S504E; SCA1; Spinocerebellar ataxia type 1 protein Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 105 SOURCE: Rabbit