CST, 2189T, P-Cadherin (C13F9) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying P-Cadherin. Validated for WB,WB,IHC,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, P-Cadherin (C13F9) Rabbit Monoclonal Antibody (CST #2189) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunohistochemistry (Paraffin): 1:100 - 1:400 Immunofluorescence (Immunocytochemistry): 1:200 Flow Cytometry (Live): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 23761 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Live) Specificity / Sensitivity P-Cadherin (C13F9) Rabbit Monoclonal Antibody recognizes endogenous levels of total P-cadherin protein. This antibody does not cross-react with other cadherin family members. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro578 of human P-cadherin protein. Background Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers (1-3). Research studies indicate that cancer cells have upregulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch." N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion (3). Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8). Alternate Names CADH3; cadherin 3; cadherin 3, type 1, P-cadherin (placental); Cadherin-3; calcium-dependent adhesion protein, placental; CDH3; CDHP; HJMD; P-cadherin; PCAD; Placental cadherin Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 120 Source/Isotype: Rabbit IgG