CST, 2197T, Phospho-Chk2 (Thr68) (C13C1) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Chk2 (Thr68) phosphate. Validated for WB,WB,IP,IHC,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Chk2 (Thr68) (C13C1) Rabbit Monoclonal Antibody (CST #2197) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:100 - 1:400 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 28130 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Phospho-Chk2 (Thr68) (C13C1) Rabbit Monoclonal Antibody detects endogenous levels of Chk2 only when phosphorylated at Thr68. Non-specific staining of mitotic cells was observed by immunohistochemistry. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr68 of human Chk2. Background Chk2 is the mammalian orthologue of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases (1-3). The amino-terminal domain of Chk2 contains a series of seven serine or threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50, and Thr68) each followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases (4,5). After DNA damage by ionizing radiation (IR), UV irradiation, or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR (5-7). The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 at residues Thr383 and Thr387 in the activation loop of the kinase domain (8). Alternate Names Cds1; Cds1 homolog; Checkpoint kinase 2; checkpoint-like protein CHK2; CHEK2; CHK2; CHK2 checkpoint homolog; CHK2 checkpoint homolog (S. pombe); hCds1; HuCds1; LFS2; PP1425; protein kinase CHK2; RAD53; Serine/threonine-protein kinase Chk2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 62 Source/Isotype: Rabbit IgG